Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged proteins for research to production of biopharmaceuticals for clinical use. Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. High level of protein expression also depends on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.
The quality of starting materials resulting from Fermentation Scale Up often determine the purification strategy or even the success of purification. Our Cell Culture Services aim at achieving maximal r-protein production through optimization of fermentation processes and it could be a very important purification step in itself. Time and effort invested at this stage often result in substantially shorter purification protocols and higher yields of active r-protein. Our experience with various high-producing cell lines, cell culture growth modes, media formulations, methods of protein extraction, fractionation and enrichment enables us to design the most optimal production process for your starting materials.
Our skills in custom protein purification were gained over many years of work with protein pharmaceuticals, drug targets, biological system components and biochemical reagents. All Protein Purification Services start with the analysis of physico-chemical and biological properties of a target protein resulting in the development of tailored procedures for its extraction, purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degradation products and other contaminants, selection of buffer conditions that stabilize biological activity and prevent product degradation. We routinely work with antibodies, antigens, enzymes, growth factors, DNA-binding proteins, membrane proteins, blood proteins and many more. Although most proteins require an individual approach, we are confident that we can handle your protein. We will purify it cost-efficiently, characterize it according to your specifications and deliver it to you in an active and application-compatible form.
SDS-PAGE and/or dot/Western blotting are routinely used for fraction analysis final protein purity is determined by densitometry from Coomassie-stained gels final products are supplied with a certificate of analysis, purification report and, if applicable, a batch record
Analytical characterization ensures the identity, purity, structural and conformational integrity, and function of the protein. We perform a number of routine protein analyses throughout all project stages. If needed, we can submit your samples for additional methods of characterization. We also offer specialized protein characterization services for purified proteins.
We offer our experience in Functional Assays & Assay Development for detailed mechanistic studies of a target protein, bioanalytical measurments and high-throughput screening. Since we thoroughly characterize the materials for which an assay is developed, our assays have maximized signal windows, low variability and are economical. These assays can have diverse applications such as analysis of enzyme activities, small molecule binding to proteins, protein-protein interactions and nucleic acid-protein interactions. In addition, we offer our services for outsourcing your assays or mechanistic studies.
Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used assay in diagnostics, drug discovery, clinical development and other life-science industries. This is a sensitive assay that detects and quantifies a target molecule in biological fluids or solutions. ELISA can be performed in various formats and platforms. Although a target-specific antibody is the central aspect of an ELISA, other components are also critical for the assay performance. Our seamless, integrated expertise in antibody-antigen biochemistry, functional assays, recombinant protein production, hybridoma cell culture, antibody purification, characterization and conjugation allows us to deliver a fully optimized and validated ELISA for its intended final application.
Labeled proteins allow us to study specific molecular interactions with high sensitivity in complex biological systems. They are important reagents in numerous biological applications such as assays, purifications, protein arrays, localization studies, flow cytometry, clinical imaging and much more. The quality of labeled proteins is critical for consistent and reliable data. Although labeling procedures appear to be simple and straightforward, most of them still need to be adjusted to take into consideration the nature of a protein in order to achieve the desired results. Possible problems during labeling procedures include protein losses due to precipitation, sample manipulation & instability, inconsistent label-to-protein ratio, incomplete removal of an unconjugated labeling probe, and poor protein characterization before and after labeling. In addition, new labeling technologies had emerged for site-directed labeling requiring an integration of protein expression and purification into the labeling process. We have extensive experience with various protein labeling techniques and are confident that we can provide you with high-quality labeled reagents for your downstream applications.
Endotoxin Removal from biological solutions is critical for many in vivo and cell-based applications, as it interferes with biological response. Endotoxins are liposaccharides that are found in the outer cell wall of Gramm-negative bacteria. Bacteria release endotoxins at the time of lysis. The toxic effect of endotoxins is triggered by its interaction with specific receptors on the immune cells resulting in the release of high concentrations of cytokines and other molecules of immunological significance. Since adventitious endotoxin is present in air, water, labware and it cannot be removed by simple sterilization, it is almost impossible to generate endotoxin-free solutions without a removal procedure. Each Endotoxin Removal project requires a protocol development step that takes into consideration biophysical properties of a target molecule, final sample application and desired formulation. For years we had successfully developed Endotoxin Removal protocols to meet strict endotoxin presence requirements. We are confident that we can make your Endotoxin Removal project a success.
In the last 30 years, Antibodies had become very important reagents in research, diagnostics, and medicine. This class of molecules are heavily used now in many fields of biomedical research such as immunoassays (Western blots, ELISA, etc.), protein purification, protein identification and protein-protein interaction studies. Many commercial diagnostic kits have antibodies as their critical components. Currently, there are 75 Monoclonal Antibodies or their derivatives approved by the FDA and many more are being tested in clinical studies. Each antibody project requires a customized approach gathering all facts about an antigen and defining the properties of the final antibody. We have a long history in developing and producing antibodies for our clients. We will plan your Antibody project thoroughly to achieve desired outcomes.