Summary of Commercial Kits and Services
Each removal technique addresses a different problem regarding endotoxins. The most prominent methods that companies have been using are based on separation by size, which uses filtration; separation by charge, which uses ion-exchange resins, separation by phase, which uses a non-ionic detergent Triton X114, and separation by affinity, which uses various ligands.
| Commercial Kit | Removal Method | Recommended Use | Company Information |
| Pierce Detoxi-Gel | Polymixin B ligand | Proteins | Thermo Fisher Scientific |
| EndoTrapÒ | Proprietary non-Polymixin B-based ligand | Proteins | Hyglos |
| AltecoÒ LPS Adsorber | Tailor-made peptide ligand | Proteins | Alteco Medical |
| ToxinEraserTM | Modified Polymixin B ligand | Proteins | Genscript |
| EtoxiClearTM | Proprietary ligand | Proteins | ProMetic BioSciences Ltd |
| ProteoSpin Endotoxin Removal | Proprietary resin | Proteins | Norgen Biotek Corp |
| BcMagTM Quick Endotoxin Removal Beads | Polymixin B ligand | Proteins | BioClone Inc |
| ProteoSpin Endotoxin Removal | Proprietary resin | Proteins | BioSynthesis |
| PyroCLEAN | Biodegradable detergent | Proteins | ALerCHEK, Inc |
| MiroCleanÒ | Organic extraction | Plasmid DNA | Mirus |
| MoBio UltraCleanÒ | Organic extraction | Plasmid DNA | Mo Bio Laboratories Inc. |
| Endotoxin Removal Kit | Organic extraction | Plasmid DNA | Lambda Biotek |
| Endotoxin Removal Kit | Detergent partitioning | Plasmid DNA | BioPioneer |
| AcrodiscÒ Unit | Anion-exchange filters | Proteins | Pall Corporation |
| EndotoxinOUT | Polymixin B ligand | Plasmid DNA | G BioSciences |
| Endotoxin Removal Beads | Polycationic ligand | Proteins | Miltenyi Biotec |
| Wizard MagneSil TfxTM System | Proprietary resin | Plasmid DNA | Promega |
| Endotoxin Removal solution | Phase partitioning by Triton X114 | Plasmid DNA | Sigma-Aldrich |
| Affi-PrepÒ Polymyxin Matrix | Polymixin B ligand | Plasmid DNA | Bio-Rad |
Although many researchers rely on various endotoxin removal kits in order to do these processes themselves, there are many factors that can effect endotoxin removal such as ligand density, pH, salt concentration, the isoelectric point, and hydrophobicity of the target molecule. ARVYS Proteins is a contract research company that offers high-quality endotoxin removal services from protein solutions. If needed, ARVYS Proteins Inc. will develop customized endotoxin removal protocols when a standard protocol cannot be applied for various reasons.
For more information, visit www.arvysproteins.com or contact us here.
