Developments in the Optimization of Recombinant Protein Expression Biological sciences owe an enormous debt to the power and utility of recombinant protein expression. It has drastically expanded the number of proteins that can be investigated both biochemically and structurally. Protein… Continue Reading
A variety of methods have been developed in order to remove endotoxins from protein solutions, with one of the more popular being separation by phase using non-ionic detergent Triton X-114. Triton X-114 detergent is homogeneous at cold temperatures, but once… Continue Reading
Summary of Commercial Kits and Services Each removal technique addresses a different problem regarding endotoxins. The most prominent methods that companies have been using are based on separation by size, which uses filtration; separation by charge, which uses ion-exchange… Continue Reading
Endotoxins are toxic protoplasms that are released when bacteria grow, die, and disintegrate. These endotoxins are lipopolysaccharides, which reside in the outer membrane of gram-negative bacteria. The gram-negative cell wall is composed of a thin, inner layer of peptidoglycan and… Continue Reading
