ӒKTA Protein Purification Systems The popularization of methods to obtain large quantities of proteins, peptides, and other large biomolecules has led to the advent of fast protein liquid chromatography (also known as fast-performance liquid chromatography or medium pressure liquid chromatography).… Continue Reading
The extraction of membrane proteins from biological membranes is usually accomplished with the use of detergents. While some general rules can be surmised, the choice of detergent best suited for the purification of a given membrane protein needs to be… Continue Reading
When working with proteins, it is essential to exercise a precise degree of control within your solutions. Proteinaceous solutions may contain many different residues from fermentation steps like cell residues, aggregates, or fragments. One of the common methods of protein… Continue Reading
Protein purification by chromatography is a technique that is familiar to anybody who’s worked in a biochemical laboratory. The concept of separating complex biological mixtures into their components holds much biophysical utility that many scientists rely on for purifying their… Continue Reading
A variety of methods have been developed in order to remove endotoxins from protein solutions, with one of the more popular being separation by phase using non-ionic detergent Triton X-114. Triton X-114 detergent is homogeneous at cold temperatures, but once… Continue Reading
