A variety of methods have been developed in order to remove endotoxins from protein solutions, with one of the more popular being separation by phase using non-ionic detergent Triton X-114. Triton X-114 detergent is homogeneous at cold temperatures, but once… Continue Reading
Summary of Commercial Kits and Services Each removal technique addresses a different problem regarding endotoxins. The most prominent methods that companies have been using are based on separation by size, which uses filtration; separation by charge, which uses ion-exchange… Continue Reading
Endotoxins are toxic protoplasms that are released when bacteria grow, die, and disintegrate. These endotoxins are lipopolysaccharides, which reside in the outer membrane of gram-negative bacteria. The gram-negative cell wall is composed of a thin, inner layer of peptidoglycan and… Continue Reading
